Enzyme Kinetics and Factors Affecting Enzyme Activity

Description

Enzyme kinetics studies the rate of enzymatic reactions, often explained by the Michaelis-Menten equation, involving substrate concentration, enzyme affinity (Km), and maximum velocity (Vmax). Enzymes catalyze reactions by lowering activation energy. Factors affecting enzyme activity include temperature, pH, substrate concentration, enzyme concentration, and inhibitors (competitive or non-competitive). Optimal temperature and pH vary by enzyme. Deviations reduce activity. Enzyme activity increases with substrate concentration until saturation. Understanding kinetics helps in drug design, metabolic control, and diagnosing enzyme-related disorders.